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Electron capture dissociation of substance P using a commercially available Fourier transform ion cyclotron resonance mass spectrometer
Author(s) -
Axelsson Jan,
Palmblad Magnus,
Håkansson Kristina,
Håkansson Per
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990330)13:6<474::aid-rcm505>3.0.co;2-1
Subject(s) - fourier transform ion cyclotron resonance , chemistry , ion cyclotron resonance , mass spectrometry , hybrid mass spectrometer , selected ion monitoring , dissociation (chemistry) , ion , cyclotron resonance , electron capture dissociation , atomic physics , fourier transform , electron cyclotron resonance , analytical chemistry (journal) , cyclotron , selected reaction monitoring , tandem mass spectrometry , physics , chromatography , gas chromatography–mass spectrometry , organic chemistry , quantum mechanics
Electron capture dissociation of the peptide Substance P is reported for the first time, with an unmodified, commercially available Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. The fragmentation pattern is compared with that obtained with collisionally induced dissociation of the ions in the electrospray ion source, and note that electron capture dissociation gives a more easily interpreted spectrum, showing mainly C ‐fragments. With the exception of the proline residues, which require cleavage of two chemical bonds, we observe all C ‐fragmental we find the bias voltage of the electron gun not to be very critical. Copyright © 1999 John Wiley & Sons, Ltd.