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The rapid identification of drug metabolites using capillary liquid chromatography coupled to an ion trap mass spectrometer
Author(s) -
Dear G. J.,
Ayrton J.,
Plumb R.,
Fraser I. J.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990315)13:5<456::aid-rcm508>3.0.co;2-t
Subject(s) - chemistry , chromatography , ion trap , mass spectrometry , electrospray , quadrupole ion trap , triple quadrupole mass spectrometer , capillary action , selected reaction monitoring , analytical chemistry (journal) , mass spectrum , tandem mass spectrometry , quadrupole mass analyzer , liquid chromatography–mass spectrometry , hybrid mass spectrometer , materials science , composite material
Capillary liquid chromatography (LC) using a 320 µm column and a flow rate of 10 µL/min has been coupled to an ion trap mass spectrometer using electrospray ionisation (ESI) to enable the rapid and effective identification of metabolites in urine, following oral administration of a novel human neutrophil elastase inhibitor, GW311616. Metabolites were identified from their mass (MS) spectra and tandem (MS/MS) mass spectra using minimal sample (1 µL of urine) and no sample pretreatment. Sensitivity assessment has shown that both molecular weight and structural information is obtainable on as little as 5 pg of compound, making the capillary LC/ion trap system as described an ideal analytical tool for the detection and characterisation of low level metabolites in biofluids (particularly when sample volume is limited). This level of detection was unattainable using a triple quadrupole mass spectrometer operating in full‐scan mode, although 200 fg on column was detected using selected reaction monitoring target analysis. Copyright © 1999 John Wiley & Sons, Ltd.