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In vivo microdialysis/liquid chromatography/tandem mass spectrometry for the on‐line monitoring of melatonin in rat
Author(s) -
Wong Philip S. H.,
Yoshioka K.,
Xie F.,
Kissinger P. T.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990315)13:5<407::aid-rcm500>3.0.co;2-i
Subject(s) - microdialysis , chemistry , chromatography , melatonin , in vivo , tandem mass spectrometry , liquid chromatography–mass spectrometry , mass spectrometry , high performance liquid chromatography , biochemistry , extracellular , medicine , microbiology and biotechnology , biology
Liquid chromatography/tandem mass spectrometry (LC/MS/MS) has been coupled to in vivo microdialysis for on‐line monitoring of melatonin in a freely moving rat for a period of 15 hours. A microdialysis probe was surgically implanted into the jugular vein of the rat, and deionized water was used as the perfusion medium at a flow rate of 1.0 µL/min. Microdialysis samples were collected in an on‐line injector with sample injection every 30 minutes. Melatonin was dosed by intraperitoneal (i.p.) injection and then monitored by microdialysis/LC/MS/MS. The whole experiment, including the microdialysis sampling and sample injection into the LC/MS system, was fully automated. Metabolites of melatonin were identified off‐line by LC/MS n experiments. Two metabolites were identified as 6‐hydroxymelatonin and cyclic 2‐hydroxymelatonin, consistent with ones found previously in the literature. Copyright © 1999 John Wiley & Sons, Ltd.