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High performance liquid chromatography quadrupole ion trap and gas chromatography/mass spectrometry studies of polyhydroxyalkaloids in bluebells
Author(s) -
Egan Martin J.,
Porter Elaine A.,
Kite Geoffrey C.,
Simmonds Monique S. J.,
Barker James,
Howells Sían
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990228)13:4<195::aid-rcm433>3.0.co;2-d
Subject(s) - chemistry , chromatography , mass spectrometry , quadrupole ion trap , ion trap , gas chromatography , trap (plumbing) , quadrupole , gas chromatography–mass spectrometry , analytical chemistry (journal) , physics , atomic physics , environmental engineering , engineering
A crudely purified polyhydroxyalkaloid (PHA) extract of Hyacinthoides non‐scripta (Hyacinthaceae) was studied by gas chromatography/mass spectrometry (GC/MS), liquid chromatography/sequential mass spectrometry (LC/MS n ) and direct infusion atmospheric pressure chemical ionisation‐mass spectrometry (APCI‐MS) to determine the best method for screening plant extracts for PHAs. Direct infusion was determined to be the technique of choice to carry out rapid screening, whereas LC/MS has the potential to reveal isomeric compounds with appropriate high performance liquid chromatography (HPLC) separation, and we report here a successful HPLC separation of underivatised PHAs in a crude extract. The requirement to derivatise PHAs for GC/MS analysis was found to confuse screening due to the formation of multiple derivatives. Also, the electron ionization (EI) mass spectra were of limited diagnostic value and GC/MS failed to detect high molecular weight PHA derivatives such as those of PHA‐diglycosides. Two glycosides not previously reported in Hyacinthaceae were detected by direct infusion APCI‐MS. Copyright © 1999 John Wiley & Sons, Ltd.