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Magnetic sector‐ion trap mass spectrometry with electrospray ionization for high sensitivity peptide sequencing
Author(s) -
Loo Joseph A.,
Muenster Helmut
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990115)13:1<54::aid-rcm450>3.0.co;2-y
Subject(s) - chemistry , ion trap , mass spectrometry , melittin , tandem mass spectrometry , electrospray ionization , analytical chemistry (journal) , ion , top down proteomics , electrospray , chromatography , extractive electrospray ionization , selected reaction monitoring , detection limit , peptide , biochemistry , organic chemistry
A hybrid mass spectrometer composed of a high resolution double focusing instrument (electrostatic analyzer‐magnetic sector, EB) and an ion trap analyzer (T) exhibits high sensitivity performance for peptide sequencing with electrospray ionization (ESI). MS 2 and MS 3 experiments for multiply charged tryptic peptides and larger peptides (e.g., melittin, 2.8 kDa) generate sequence‐informative product ions. Collisionally activated dissociation (CAD) of selected precursor ions can also be performed in the interface between the double focusing analyzer and the ion trap (transfer octapole region) to generate product ions. With a low‐flow micro‐ESI source, which can deliver analyte solution to the source at a flowrate of 10–200 nL/min, tandem mass spectra can be obtained from sub‐fmol amounts of melittin. The high resolving power of the MS‐I stage combined with the efficiency of the ion trap stage allows for high resolution precursor ion selection with subsequent highly sensitive tandem mass spectrometry (MS/MS) analysis.© 1999 John Wiley & Sons, Ltd.