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Malononitrile as a New Derivatizing Reagent for High‐sensitivity Analysis of Oligosaccharides by Electrospray Ionization Mass Spectrometry
Author(s) -
Ahn Yeong Hee,
Yoo Jong Shin
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19981230)12:24<2011::aid-rcm429>3.0.co;2-r
Subject(s) - chemistry , derivatization , malononitrile , reagent , chromatography , electrospray ionization , detection limit , mass spectrometry , organic chemistry , catalysis
A new method for the high‐sensitivity analysis of oligosaccharides by negative ion electrospray ionization mass spectrometry was developed through a chemical derivatization of oligosaccharides. Oligosaccharides were derivatized to dinitrile compounds from the reaction with malononitrile under mildly basic conditions. The derivative of maltoheptaose was detected mainly as the [M‐2H] 2− ion in negative ion mode with 20 fmol sensitivity, even in unpurified samples. In this malononitrile derivatization method, no inorganic reagent, other than sodium hydroxide as a base catalyst, is used. Also, because excess ligand (malononitrile) is volatile, high sensitivity detection is realized without any solvent extraction or chromatographic purification. The detection limit can also be decreased by simple on‐line cartridge filtration to 200 attomol which is 10 5 times better than that of free maltoheptaose. Structural information for oligosaccharide derivatives was obtained by collision induced dissociation. This malononitrile derivatization method is convenient and efficient for the sensitive analysis of oligosaccharides. Copyright © 1998 John Wiley & Sons, Ltd.