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Identification of drug metabolites in biological matrices by intelligent automated liquid chromatography/ tandem mass spectrometry
Author(s) -
Lopez Linda L.,
Yu Xiao,
Cui Donghui,
Davis Margaret R.
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19981130)12:22<1756::aid-rcm381>3.0.co;2-c
Subject(s) - chemistry , chromatography , tandem mass spectrometry , liquid chromatography–mass spectrometry , mass spectrometry , metabolite , indinavir , human immunodeficiency virus (hiv) , biochemistry , medicine , sida , family medicine , viral disease
A rapid and systematic strategy for the identification of drug metabolites in biological matrices based on liquid chromatography ‐ tandem mass spectrometry (LC/MS/MS) techniques was utilized for the identification of drug metabolites of the HIV protease inhibitor Indinavir. This strategy integrates intelligent real‐time mass spectrometry with HPLC detection and a predictive strategy for detecting metabolites arising from common biotransformations, to rapidly elucidate structures of drug metabolites. Structures of metabolites generated from in vitro incubation mixtures of Indinavir were characterized from a single chromatographic analysis using the automated LC/MS/MS methodology, thus reducing data acquisition time and improving efficiency. © 1998 John Wiley & Sons, Ltd.