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Monitoring the hybridization of the components of oligonucleotide mixtures to immobilized DNA via matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Bleczinski Colleen F.,
Richert Clemens
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19981130)12:22<1737::aid-rcm393>3.0.co;2-4
Subject(s) - chemistry , oligonucleotide , histone octamer , mass spectrometry , dna , chromatography , desorption , matrix (chemical analysis) , analytical chemistry (journal) , biochemistry , organic chemistry , adsorption , histone , nucleosome
Reported here is how the hybridization of individual components of oligonucleotide mixtures to solid‐phase bound complementary strands can be monitored simultaneously by quantitative matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS). Three oligonucleotides, a DNA heptamer, a DNA octamer and a DNA octamer with a terminal cholic acid appendage were used as the test mixture. Upon cooling in the presence of a complementary undecamer on controlled pore glass, depletion of the components from the solution was observed. The resulting hybridization curves show the same relative affinities as traditional UV melting curves with single components and their complement. Assays of the kind described here may be used to select high affinity binders from combinatorial libraries of modified antisense oligonucleotides. © 1998 John Wiley & Sons, Ltd.