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Quantitative determination of the angiotensin‐converting enzyme inhibitor lisinopril in human plasma by stable isotope dilution gas chromatography/negative ion chemical ionization mass spectrometry
Author(s) -
Leis H. J.,
Fauler G.,
Raspotnig G.,
Windischhofer W.
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19981115)12:21<1591::aid-rcm368>3.0.co;2-c
Subject(s) - chemistry , isotope dilution , chromatography , lisinopril , mass spectrometry , chemical ionization , gas chromatography , ion , plasma , ionization , angiotensin converting enzyme , organic chemistry , medicine , radiology , blood pressure , physics , quantum mechanics
A simple, highly accurate and precise method for the quantitative measurement of the angiotensin‐converting enzyme inhibitor lisinopril in human plasma is presented. The assay is based on gas chromatography/negative ion chemical ionization mass spectrometry. The preparation of stable isotope labelled lisinopril for use as an internal standard is described. The method involves solid phase extraction on C18 sorbent and derivatization to the methyl diester–trifluoroacetamide derivatives. The detection limit was found to be 50 pg and a lower limit of quantification was reached down to 0.5 ng/mL plasma. © 1998 John Wiley & Sons, Ltd.