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Capillary chromatography/microelectrospray mass spectrometry used for the identification of putative cyclin‐dependent kinase inhibitory protein in Medicago
Author(s) -
Kele Zoltán,
Janáky Tamás,
Mészáros Tamás,
Fehér Attila,
Dudits Dénes,
Szabó Pál T.
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19981030)12:20<1564::aid-rcm351>3.0.co;2-t
Subject(s) - chemistry , chromatography , mass spectrometry
A home‐built capillary chromatography/microelectrospray system was used for peptide mass mapping of a putative cyclin‐dependent kinase inhibitor protein with molecular mass of 8.5 kDa. The masses of identified tryptic fragments were then input to a protein database search routine. Daughter ion scans were done only on the most abundant tryptic fragments. On the basis of protein database search results and tandem mass spectrometric measurements the bioactive protein was identified as ubiquitin. © 1998 John Wiley & Sons, Ltd.