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A new linear ion trap time‐of‐flight system with tandem mass spectrometry capabilities
Author(s) -
Campbell J. M.,
Collings B. A.,
Douglas D. J.
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19981030)12:20<1463::aid-rcm357>3.0.co;2-h
Subject(s) - chemistry , ion trap , quadrupole ion trap , ion , mass spectrometry , analytical chemistry (journal) , atomic physics , time of flight mass spectrometry , excitation , time of flight , hybrid mass spectrometer , tandem mass spectrometry , fragmentation (computing) , selected reaction monitoring , ionization , physics , organic chemistry , chromatography , quantum mechanics , computer science , operating system
A new hybrid linear ion trap time‐of‐flight mass spectrometer has been constructed and the first results for tandem‐in‐time MS/MS in a 2‐dimensional Paul trap are presented. The instrument consists of an electrospray ionization source and two radio frequency (RF) only quadrupoles operating at a pressure of 7 mTorr N 2 which are coupled orthogonally to a linear TOFMS. Ions are trapped in an RF‐only quadrupole through the application of timed stopping potentials on the entrance and exit apertures. Through the coupling of an auxiliary waveform generator on one pair of the ion trap rods a small dipolar excitation voltage is superimposed on the quadrupolar electric field. This voltage is used for precursor isolation via broadband excitation and collision induced dissociation through mass selective resonant excitation. Instrumental performance and choice of operating parameters are demonstrated by recording fragment spectra for +3 ions of renin substrate and +1 ions of reserpine. TOFMS mass resolution ( m /Δ m ) FWHM is as high as 740 at m / z = 609. Fragmentation efficiency is greater than 50% and excitation mass resolution is 60–75. © 1998 John Wiley & Sons, Ltd.