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Microfabricated device coupled with an electrospray ionization quadrupole time‐of‐flight mass spectrometer: protein identifications based on enhanced‐resolution mass spectrometry and tandem mass spectrometry data
Author(s) -
Figeys Daniel,
Lock Chris,
Taylor Lorne,
Aebersold Ruedi
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19981030)12:20<1435::aid-rcm349>3.0.co;2-9
Subject(s) - chemistry , mass spectrometry , protein mass spectrometry , tandem mass spectrometry , selected reaction monitoring , hybrid mass spectrometer , top down proteomics , electrospray ionization , resolution (logic) , analytical chemistry (journal) , quadrupole mass analyzer , ion mobility spectrometry–mass spectrometry , triple quadrupole mass spectrometer , quadrupole time of flight , quadrupole , sample preparation in mass spectrometry , chromatography , atomic physics , physics , artificial intelligence , computer science
We describe the coupling of a microfabricated fluidic device to an electrospray ionization (ESI) quadrupole time‐of‐flight mass spectrometer (QqTOFMS) for the identification of protein samples. The microfabricated devices consisted of three reservoirs connected via channels to a main capillary, which in turn was linked via a microspray interface to the QqTOFMS. Here we present preliminary results obtained using this system. Standardized solutions of myoglobin tryptic digest were analyzed indicating a limit of detection at the low to sub fmol/μL. The combination of the microfabricated device for rapid sample delivery and the rapid acquisition capability, enhanced resolution and mass accuracy of the QqTOF offers unique possibilities for the rapid identification of proteins by database searching. This platform can generate MS data suitable for protein database searching by the peptide‐mass fingerprinting approach and MS/MS data suitable for protein database searching. Here the results of the two database‐searching approaches are compared and the possibilities of combining the two approaches for rapid identification of protein are discussed. Also, we present a comparison of the results obtained using the three‐position microfabricated device coupled to the ESI‐QqTOFMS and to an ESI‐ion trap MS. Finally the combination of C‐terminal 18 O labeling of peptides and the microfabricated system for automated combined peptide‐mass fingerprinting and sequence‐tag database searching is discussed. © 1998 Crown Copyright, Canada