Premium
Differentiation of lysine/glutamine in peptide sequence analysis by electrospray ionization sequential mass spectrometry coupled with a quadrupole ion trap
Author(s) -
Bahr U.,
Karas M.,
Kellner R.
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19981015)12:19<1382::aid-rcm327>3.0.co;2-j
Subject(s) - chemistry , electrospray ionization , mass spectrometry , fragmentation (computing) , isobaric process , ion trap , quadrupole ion trap , peptide , ion , electrospray , glutamine , top down proteomics , isobaric labeling , protein mass spectrometry , chromatography , lysine , collision induced dissociation , quadrupole , tandem mass spectrometry , amino acid , biochemistry , organic chemistry , atomic physics , physics , computer science , thermodynamics , operating system
Electrospray ionization coupled to a quadrupole ion trap mass spectrometer is used to differentiate between the isobaric amino acids lysine and glutamine in sequence analysis of peptides. Collision‐induced dissociation is used for fragmentation. Several isobaric peptides with one or more lysines or glutamines at different positions were investigated. The ambiguous amino acid either in the peptide chain or at the C‐ or N‐terminus can be clearly identified based on specific side chain fragment ions resulting from MS 3 or MS 4 of B‐ and Y″‐fragment ions. © 1998 John Wiley & Sons, Ltd.