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Static time‐of‐flight secondary ion mass spectrometry imaging of freeze‐fractured, frozen‐hydrated biological membranes
Author(s) -
Pacholski Michaeleen L.,
Can Donald M.,
Ewing Andrew G.,
Winograd Nicholas
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980930)12:18<1232::aid-rcm319>3.0.co;2-g
Subject(s) - chemistry , liposome , membrane , phospholipid , secondary ion mass spectrometry , mass spectrometry , time of flight , ion , chromatography , red blood cell , biophysics , analytical chemistry (journal) , biochemistry , organic chemistry , biology
The study of cell membrane lipid and steroid composition and distribution is important for the understanding of membrane dynamics and function. Here we present efforts to chemically image phospholipid distributions on a submicron scale on freeze‐fractured and frozen‐hydrated liposomes and red blood cells using time‐of‐flight secondary ion mass spectrometry. Sample preparation by freeze fracturing of membranes is described. Fragments representative of phospholipid headgroups are found to be localized on both liposomes and red blood cells. In addition, the cholesterol molecular ion [M+H] is localized on liposome surfaces. © 1998 John Wiley & Sons, Ltd.