Premium
A new ultra‐high throughput method for characterizing combinatorial libraries incorporating a multiple probe autosampler coupled with flow injection mass spectrometry analysis
Author(s) -
Wang T.,
Zeng L.,
Strader T.,
Burton L.,
Kassel Daniel B.
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980831)12:16<1123::aid-rcm293>3.0.co;2-w
Subject(s) - microtiter plate , chemistry , chromatography , mass spectrometry , sample (material) , flow injection analysis , throughput , analytical chemistry (journal) , computer science , detection limit , telecommunications , wireless
A new method for ultra‐fast flow injection analysis‐mass spectrometry (FIA‐MS) has been developed. An eight probe autosampler system implemented for FIA‐MS analysis of combinatorial libraries synthesized in microtiter plates has enabled sample analysis times to be dramatically reduced as compared to microtiter plate analyses using single arm autosamplers. Compounds contained within the microtiter plate wells are processed eight at a time, eight samples in under a minute. Using this multiple probe autosampler, it has been possible to process a single microtiter plate in 12 minutes. To facilitate the data acquisition and processing of the samples, the sample data acquisition list, originally generated for registration of compounds synthesized in a microtiter plate format, was modified using an Excel macro into the format of an injection sample list for mass spectral data acquisition. Further, an automated data processing script was developed to process the multi‐injection analysis results. Applications of the probe multiple autosampler to the analysis of combinatorial libraries synthesized in microtiter plates and compounds purified into deep well microtiter plates is presented. © 1998 John Wiley & Sons, Ltd.