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Unambiguous bead decoding by microelectrospray capillary electrochromatography tandem mass spectrometry of dansylated secondary amine tags from encoded combinatorial organic synthesis
Author(s) -
Lane Stephen J.,
Pipe Adrian
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980615)12:11<667::aid-rcm213>3.0.co;2-s
Subject(s) - chemistry , capillary electrochromatography , mass spectrometry , triple quadrupole mass spectrometer , chromatography , tandem mass spectrometry , analytical chemistry (journal) , ion mobility spectrometry–mass spectrometry , quadrupole , capillary electrophoresis , selected reaction monitoring , physics , atomic physics
Capillary electrochromatography (CEC) has been coupled to a triple quadrupole mass spectrometer for the unambiguous decoding of active single beads from encoded combinatorial organic synthesis. The high separation efficiency and plug flow profile of CEC coupled with the inherent specificity of a triple quadrupole mass spectrometer operating in the parent ion scanning mode allowed a highly specific and sensitive method to be developed for the unambiguous identification of a series of surrogate tags at the low femtomole level. We believe this is the first report of a CEC/MS/MS parent ion method and is considered complementary to the traditional decode method of high performance liquid chromatography with fluorescence detection in instances where further specificity is required. © 1998 John Wiley & Sons, Ltd.