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A new procedure for quantitative correction of matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric response
Author(s) -
Scamporrino Emilio,
Maravigna Pietro,
Vitalini Daniele,
Mineo Placido
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980529)12:10<646::aid-rcm208>3.0.co;2-c
Subject(s) - chemistry , mass spectrometry , mass spectrum , ionization , analytical chemistry (journal) , time of flight mass spectrometry , desorption , matrix assisted laser desorption/ionization , matrix (chemical analysis) , ion , molar mass , chromatography , organic chemistry , adsorption , polymer
A simple procedure for the correction of the decreasing detection response in a matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) mass spectrum with increasing ion mass is reported. On the basis of the experimental MALDI‐TOF mass spectrum of a mixture of four polymethylmethacrylate (PMMA) standards (molecular masses in the range 4–100 kDa), present in equal molar abundance, an equation describing the MALDI‐TOF response has been derived. This equation has been used to correct any subsequently recorded MALDI spectrum. As an example, the procedure has been applied here to normalize the MALDI‐TOF mass spectrum of a 1,20‐di(bisphenoxy‐A)eicosane based copolyformal ( I ) containing porphyrin units in the main chain. © 1998 John Wiley & Sons, Ltd.