Premium
Analysis of bacteriohopanepolyols in sediment and bacterial extracts by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry
Author(s) -
Fox Paul A.,
Carter James,
Farrimond Paul
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980529)12:10<609::aid-rcm210>3.0.co;2-u
Subject(s) - chemistry , atmospheric pressure chemical ionization , chromatography , chemical ionization , mass spectrometry , dichloromethane , column chromatography , environmental chemistry , analytical chemistry (journal) , ionization , ion , organic chemistry , solvent
We report the new application of negative ion atmospheric pressure chemical ionization (APCI) to the detection of composite bacteriohopanepolyols in bacterial and sedimentary (or other natural environmental) samples. The method employs the addition of dichloromethane to the high performance liquid chromatography eluent prior to APCI to stimulate formation of chlorine addition/association ions from the hopanoid alcohols. This approach provided the first direct analysis of composite bacterial hopanoids in a sediment, identifying bacteriohopanetrol and bacteriohopanetetrol cyclitol ether. © 1998 John Wiley & Sons, Ltd.