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Rapid analysis of steroidal saponin mixture using electrospray ionization mass spectrometry combined with sequential tandem mass spectrometry
Author(s) -
Fang Shiping,
Hao Chunyan,
Sun Weixing,
Liu Zhiqiang,
Liu Shuying
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980529)12:10<589::aid-rcm200>3.0.co;2-q
Subject(s) - chemistry , chromatography , protein mass spectrometry , mass spectrometry , electrospray ionization , sample preparation in mass spectrometry , tandem mass spectrometry , extractive electrospray ionization , direct electron ionization liquid chromatography–mass spectrometry interface , top down proteomics , selected reaction monitoring , saponin , analytical chemistry (journal) , ionization , chemical ionization , organic chemistry , ion , medicine , alternative medicine , pathology
Using electrospray ionization mass spectrometry (MS) combined with sequential tandem MS(ESI‐MS n ), two major steroidal saponins extracted from Tribulus terrestris were studied, and considerable useful structural information was obtained. The structure of the proposed known steroidal saponin was verified, and the structure of the unknown saponin was investigated using MS n experiments. Some special fragment ions were also observed, and the corresponding fragmentation mechanisms were investigated which are characteristic for steroidal saponins and can give some information on the linkage position of some sugar groups in saponins. This methodology has been established as a powerful tool for the rapid, comparative analysis of mixtures such as crude plant extracts. © 1998 John Wiley & Sons, Ltd.