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Application of size exclusion chromatography matrix‐assisted laser desorption/ionization time‐of‐flight to the determination of molecular masses in polydisperse polymers
Author(s) -
Montaudo Maurizio S.,
Puglisi Concetto,
Samperi Filippo,
Montaudo Giorgio
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980515)12:9<519::aid-rcm186>3.0.co;2-l
Subject(s) - chemistry , dispersity , size exclusion chromatography , analytical chemistry (journal) , polymer , mass spectrometry , polydimethylsiloxane , chromatography , molar mass distribution , fractionation , desorption , gel permeation chromatography , matrix assisted laser desorption/ionization , time of flight mass spectrometry , ionization , polymer chemistry , ion , organic chemistry , adsorption , enzyme
The determination of molecular mass (MM) data for polydisperse polymers by size exclusion chromatography matrix assisted laser desorption/ionization time‐of‐flight (SEC/MALDI‐TOF) involves the fractionation of samples through an analytical SEC. Selected fractions are then analysed by MALDI‐TOF and the mass spectra of these nearly monodisperse samples allow the determination of M n and M w averages. To test the reliability of the molecular mass estimates by the SEC/MALDI‐TOF method, a sample of polymethylmethacrylate (PMMA), two samples of polydimethylsiloxane (PDMS), and four samples of copolyesters, all polydisperse, were analysed. The results show that the molecular mass values of PMMA fractions obtained by MALDI‐TOF are coincident with those obtained using the SEC calibration plots obtained with anionic PMMA standards. In the case of the two polydimethylsiloxanes (PDMS1 and PDMS2: linear and cyclic, respectively), two slightly differing SEC calibration plots were obtained, reflecting the different structures of the polymer chains of the two samples. The SEC traces of four copolyesters were obtained in tetrahydrofuran and CHCl 3 . Data on MM, MM distribution solvent effects and copolymer composition are reported. © 1998 John Wiley & Sons, Ltd.