Premium
Investigation of spectral reproducibility in direct analysis of bacteria proteins by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Wang Zhengping,
Russon Larry,
Li Liang,
Roser Dennis C.,
Long S. Randolph
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980430)12:8<456::aid-rcm177>3.0.co;2-u
Subject(s) - reproducibility , chemistry , mass spectrometry , mass spectrum , analytical chemistry (journal) , sample preparation , matrix (chemical analysis) , chromatography , matrix assisted laser desorption/ionization , ionization , surface enhanced laser desorption/ionization , desorption , sample preparation in mass spectrometry , electrospray ionization , ion , organic chemistry , adsorption
Matrix‐assisted laser desorption/ionization (MALDI) time‐of‐flight mass spectrometry (TOFMS) can be used for rapid detection of bacteria proteins in a crude mixture. It can potentially be used as a tool for bacterial identification based on the mass spectral patterns or the appearance of some characteristic mass peaks. However, there are many experimental parameters that can potentially have a strong effect on the observed mass spectra. The objective of this work is to address the mass spectral reproducibility issue. Several experimental parameters that may affect the MALDI spectra are systematically investigated. Results of spectral comparison from two laboratories with different operators and instrumentation are presented. It is demonstrated that minor variations in the sample/matrix preparation procedures for MALDI and in the experimental conditions used for bacterial protein extraction can result in a significant change in the observed spectra, though a number of peaks are conserved in the spectra obtained under different experimental conditions from the same bacterial sample. These conserved peaks may potentially be used as the biomarkers for bacterial identification. It is stressed that this type of investigation on spectral reproducibility should be carried out for different bacterial species in order to identify the mass spectral peaks that are consistently detected regardless of operator and nominal variations in sample preparation approach. © 1998 John Wiley & Sons, Ltd.