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Monitoring of liquid‐phase peptide synthesis on soluble polymer supports via matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Thürmer R.,
Meisenbach M.,
Echner H.,
Weiler A.,
AlQawasmeh R. A.,
Voelter W.,
Korff U.,
SchmittSody W.
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980415)12:7<398::aid-rcm171>3.0.co;2-8
Subject(s) - chemistry , mass spectrometry , desorption , sample preparation in mass spectrometry , polyethylene glycol , chromatography , matrix assisted laser desorption/ionization , polymer , ionization , matrix (chemical analysis) , protein mass spectrometry , peptide , peptide synthesis , analytical chemistry (journal) , organic chemistry , tandem mass spectrometry , electrospray ionization , adsorption , ion , biochemistry
Taking the enkephalin synthesis on soluble polyethylene glycol support as a test case, this communication demonstrates the usefulness of matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry to monitor each step of coupling as well as removal of the N ‐terminal protecting group of the polymer‐bound growing peptide chain. With minute amounts of sample the high mass accuracy permits detection of side reactions as well as incomplete reaction products. © 1998 John Wiley & Sons, Ltd.