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De novo peptide sequencing in an ion trap mass spectrometer with 18 O labeling
Author(s) -
Qin Jun,
Herring Christopher J.,
Zhang Xiaolong
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980314)12:5<209::aid-rcm141>3.0.co;2-s
Subject(s) - chemistry , mass spectrometry , ion trap , hybrid mass spectrometer , chromatography , quadrupole ion trap , quadrupole mass analyzer , mass spectrum , top down proteomics , triple quadrupole mass spectrometer , peptide , analytical chemistry (journal) , capillary action , selected reaction monitoring , tandem mass spectrometry , biochemistry , materials science , composite material
De novo peptide sequencing in an ion trap mass spectrometer coupled on‐line with a capillary HPLC using 18 O labeling provides a viable alternative to the method using the combination of nanospray, 18 O labeling and a quadrupole/time‐of‐flight mass spectrometer. Seven to sixteen amino acid residues can be sequenced from the liquid chromatography/randem mass spectrometry (LC/MS/MS) spectra. This approach combines the benefit of capillary LC and the high sensitivity of the ion trap operated in the MS/MS mode. The wide availability of the LCQ mass spectrometer makes this approach readily adaptable to the biological mass spectrometry community. © 1998 John Wiley & Sons, Ltd.