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Analysis of flavonoids in tablets and urine by gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry
Author(s) -
Watson D. G.,
Pitt A. R.
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980227)12:4<153::aid-rcm122>3.0.co;2-4
Subject(s) - chemistry , chromatography , mass spectrometry , detection limit , kaempferol , electrospray ionization , gas chromatography , ginkgo biloba , gas chromatography–mass spectrometry , liquid chromatography–mass spectrometry , quercetin , antioxidant , organic chemistry , medicine , pharmacology
A method was developed for the analysis and characterization of quercetin and kaempferol in urine following ingestion of Ginkgo biloba tablets. The method utilized gas chromatography/negative ion chemical ionization mass spectrometry of the trimethysilyl derivatives of the flavonoids. Limits of detection for these compounds using this method were ca . 20 pg on column. Liquid chromatography with electrospray mass spectrometry in the negative ion mode was utilized to characterize the complex mixture of glycosides present in the G. biloba tablets, the limit of detection with this technique was ca . 10 ng on column. © 1998 John Wiley & Sons, Ltd.