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Study of erythromycin A decomposition products in aqueous solution by solid‐phase microextraction/liquid chromatography/tandem mass spectrometry
Author(s) -
Volmer Dietrich A.,
Hui Joseph P. M.
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980214)12:3<123::aid-rcm126>3.0.co;2-4
Subject(s) - chemistry , chromatography , tandem mass spectrometry , mass spectrometry , solid phase microextraction , aqueous solution , electrospray ionization , electrospray , extraction (chemistry) , sample preparation in mass spectrometry , liquid chromatography–mass spectrometry , gas chromatography–mass spectrometry , organic chemistry
The decomposition of erythromycin A (EA) in aqueous solution was examined in the pH range 2–13 by means of combined solid‐phase microextraction (SPME) and liquid chromatography/electrospray ionization tandem mass spectrometry. Degradation of EA, especially at lower pH values (pH ≤ 3), was very rapid and yielded a wide variety of decomposition products. Identification of these degradation products was achieved by means of tandem mass spectrometry in the product ion and precursor ion scan modes. Anhydroerythromycin A was shown to be the major reaction product in both acidic and basic solutions. Among the different SPME fibers investigated for extraction from aqueous solutions, polydimethylsiloxane/divinylbenzene exhibited the best performance for EA and its degradation products. © 1998 John Wiley & Sons, Ltd.