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Detection of eritadenine in extracts from Shiitake mushroom by gas chromatography/mass spectrometry
Author(s) -
Vitányi G.,
Lelik L.,
BihátsiKarsai É.,
Lefler J.,
NagyGasztonyi M.,
Vereczkey G.
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980214)12:3<120::aid-rcm125>3.0.co;2-g
Subject(s) - chemistry , mushroom , chromatography , mass spectrometry , silylation , gas chromatography , extraction (chemistry) , gas chromatography–mass spectrometry , electron ionization , ionization , organic chemistry , food science , catalysis , ion
The combination of gas chromatography and mass spectrometry (GC/MS), using an apolar capillary column and electron impact ionization (EI+), made it possible to determine the eritadenine content of Shiitake mushroom after silylation at microgram levels. The sample preparation consisted of only two steps, namely extraction of eritadenine from the mushroom and silylation with N‐methoxy‐N,O‐bistrimethylsilyl carbamate (BSMOC) at 80°C. This simple and rapid method has good precision and accuracy, is useful for quantitation of eritadenine content in caps, stems and mycelium of different species of mushrooms. © 1998 John Wiley & Sons, Ltd.