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High throughput liquid chromatography/mass spectrometry bioanalysis using 96‐well disk solid phase extraction plate for the sample preparation
Author(s) -
Simpson Heidi,
Berthemy Antoine,
Buhrman Deborah,
Burton Regina,
Newton John,
Kealy Michael,
Wells David,
Wu Danlin
Publication year - 1998
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19980131)12:2<75::aid-rcm112>3.0.co;2-c
Subject(s) - bioanalysis , chromatography , chemistry , solid phase extraction , sorbent , elution , sample preparation , extraction (chemistry) , mass spectrometry , adsorption , organic chemistry
Abstract The 96 well solid phase extraction (SPE) operated by robot in the LC/MS/MS bioanalysis offered rapid sample preparation for drugs and metabolites in biological matrices, based on simultaneous extraction of 96 samples. The use of a disk as sorbent in the 96 well plate further improved the performance of SPE and allowed for small elution volumes, making it possible to ‘dilute and shoot’ after SPE elution. In this study, a 96 well plate (Empore TM ) was developed, characterized and optimized for several pharmaceutical compounds. In addition, a robot (MultiProbe TM ) was modified to automate the 96 well plate operation. Examples were given to illustrate the major differences of using 96 well disk plate SPE in the method development as compared to the traditional SPE. This technology has been successfully used to support many clinical studies. Typically, a batch of 96 samples were prepared in 1–1.5 hours unattended (except for the replacement of a collection plate). Considerable savings in disposable supplies were also noted. © 1998 John Wiley & Sons, Ltd.