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Stable isotope ratio analysis of amino acids: the use of N ( O,S )‐ethoxycarbonyl trifluoroethyl ester derivatives and gas chromatography/mass spectrometry
Author(s) -
Pietzsch Jens,
Julius Ulrich,
Hanefeld Markolf
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199711)11:17<1835::aid-rcm76>3.0.co;2-6
Subject(s) - chemistry , mass spectrometry , chromatography , amino acid , gas chromatography , gas chromatography–mass spectrometry , pyridine , hydrolysate , organic chemistry , hydrolysis , biochemistry
N ( O,S )‐Ethoxycarbonyl trifluoroethyl amino acid esters are formed by the reaction of amino acids with ethylchloroformate plus trifluoroethanol plus pyridine. The use of these derivatives for a rapid and sensitive determination of specific enrichment of stable isotopically labeled tracer amino acids in blood plasma and protein hydrolysates, by using gas chromatography/electron impact mass spectrometry, is discussed. © 1997 John Wiley & Sons, Ltd.