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Development of a capillary high‐performance liquid chromatography tandem mass spectrometry system using SWIFT technology in an ion trap/reflectron time‐of‐flight mass spectrometer
Author(s) -
He Ling,
Wu JingTao,
Parus Steve,
Lubman David M.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19971030)11:16<1739::aid-rcm77>3.0.co;2-3
Subject(s) - chemistry , reflectron , mass spectrometry , chromatography , ion trap , top down proteomics , tandem mass spectrometry , analytical chemistry (journal) , swift , sample preparation in mass spectrometry , tandem , selected reaction monitoring , ion mobility spectrometry–mass spectrometry , time of flight mass spectrometry , ion , aerospace engineering , electrospray ionization , organic chemistry , ionization , engineering , computer science , programming language
In this work a capillary high‐performance liquid chromatography/tandem mass spectrometry system has been developed in a quadrupole ion trap/reflectron time‐of‐flight mass spectrometer. The broadband excitation for isolating a precursor ion is achieved by generating the stored waveform inverse Fourier transform waveform in real time. Following the isolation and cooling periods, an auxiliary AC voltage (tickle) is applied to the entrance end cap of the ion trap to generate collision induced dissociation. The amplitude of the tickle voltage is optimized on‐line using a feedback method which involves having two computers communicating with each other. This system has been tested with a 4‐peptide mixture and tandem mass spectra were obtained for each peptide. © 1997 John Wiley & Sons, Ltd.