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Rapid simultaneous analysis of prostaglandin E 2 , 12‐hydroxyeicosatetraenoic acid and arachidonic acid using high performance liquid chromatography/electrospray ionization mass spectrometry
Author(s) -
Newby Craig S.,
Mallet Anthony L.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19971015)11:15<1723::aid-rcm71>3.0.co;2-k
Subject(s) - chemistry , chromatography , hydroxyeicosatetraenoic acid , mass spectrometry , derivatization , arachidonic acid , electrospray ionization , electrospray , high performance liquid chromatography , quantitative analysis (chemistry) , detection limit , enzyme , biochemistry
Arachidonic acid (AA) can be metabolized to a variety of lipid mediators including prostaglandins (PGE), and hydroxyeicosatetraenoic acids (HETE) by cyclooxygenase, lipoxygenase and cytochrome P450‐dependent monooxygenase enzymatic pathways. Traditional experimental procedures to quantify these lipid mediators require purification, often by high performance liquid chromatography (HPLC), prior to derivatization for gas chromatography/mass spectrometry (GC/MS) analysis. This paper describes a rapid and simple technique for the simultaneous quantitative analysis of PGE 2 , 12‐HETE, and AA by HPLC/electrospray ionization mass spectrometry on cultured human dermal fibroblast supernatants. Extension of the method to analyse 5‐HETE and 15‐HETE was investigated. The advantages of this method include minimal sample preparation and elimination of the problem associated with thermal stability for GC/MS analysis. A detection limit of 20pg on column for PGE 2 and 5pg on column for 12‐HETE and AA was determined. © 1997 John Wiley & Sons, Ltd.