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Orthogonal acceleration single‐pass time‐of‐flight mass spectrometry for determination of the exact masses of product ions formed in tandem mass spectrometry experiments
Author(s) -
Keough T.,
Lacey M. P.,
Ketcha M. M.,
Bateman R. H.,
Green M. R.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19971015)11:15<1702::aid-rcm73>3.0.co;2-l
Subject(s) - chemistry , mass spectrometry , mass , ion , analytical chemistry (journal) , selected reaction monitoring , acceleration , ion trap , time of flight mass spectrometry , tandem mass spectrometry , hybrid mass spectrometer , isobaric process , time of flight , chromatography , mass spectrum , physics , organic chemistry , classical mechanics , ionization , thermodynamics
A hybrid magnetic sector/orthogonal acceleration single‐pass time‐of‐flight (oaTOF) mass spectrometer has been used to measure the exact masses of product ions formed in high‐ and low‐collision‐energy MSMS experiments. Methods for both external and internal calibration of the mass scale have been evaluated. Mass accuracies of ±25 ppm are routinely achieved for product ions having masses greater than 200 Da. Best case mass accuracies of ±10 ppm have been obtained. Mass accuracy at low mass is limited by the current speed of the time‐to‐digital converter (TDC). Experiments at reduced accelerating voltages suggest that doubling the TDC acquisition rate will increase low‐mass accuracy six‐fold. A number of applications of exact mass MSMS are presented, including: characterization of a side‐reaction product formed in a combinatorial library synthesis, differentiation of isobaric residues in small peptides and a ‘mimicked’ MS 3 measurement to confirm the identity of an unusual product ion previously observed in an ion trap MSMS experiment. © 1997 John Wiley & Sons, Ltd.