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Fragmentation of complex carbohydrates following ionization by matrix‐assisted laser desorption with an instrument fitted with time‐lag focusing
Author(s) -
Naven T. J. P.,
Harvey D. J.,
Brown J.,
Critchley G.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19971015)11:15<1681::aid-rcm34>3.0.co;2-0
Subject(s) - chemistry , glycosidic bond , fragmentation (computing) , ion , ionization , analytical chemistry (journal) , mass spectrum , mass spectrometry , desorption , chromatography , organic chemistry , adsorption , computer science , enzyme , operating system
Matrix‐assisted laser desorption/ionization time‐of‐flight spectra of carbohydrates recorded with an instrument using time‐lag focusing (delayed extraction) were found to exhibit fragment ions, whereas, in the non time‐lag‐focusing mode, fragment ions were not observed. Fragment ions consisted both of glycosidic and cross‐ring cleavage products whose absolute, but not relative abundance increased with the delay time and indicated that they were formed within the ion source. Only the glycosidic cleavage ions were present in post‐source decay spectra and their abundance was inversely correlated with the delay time. The results indicated that the cross‐ring cleavages were predominately the products of fast reactions whereas the glycosidic cleavages occurred over a longer time frame. © 1997 John Wiley & Sons, Ltd.