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Determination of the Chloropropanols, 3‐Chloro‐1,2‐propandiol and 2‐Chloro‐1,3‐propandiol, in Hydrolysed Vegetable Proteins and Seasonings by Gas Chromatography/Ion Trap Tandem Mass Spectrometry
Author(s) -
Hamlet Colin G.,
Sutton Peter G.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19970830)11:13<1417::aid-rcm986>3.0.co;2-s
Subject(s) - chemistry , chromatography , mass spectrometry , tandem mass spectrometry , ion trap , gas chromatography , gas chromatography/tandem mass spectrometry , gas chromatography–mass spectrometry , hydrolysis , analytical chemistry (journal) , organic chemistry
This paper reports on the development of a highly selective method for the determination of parts‐per‐billion (μg/kg) levels of 3‐chloro‐1,2‐propandiol (3‐MCPD) in hydrolysed vegetable proteins (HVP) using gas chromatography/ion trap mass spectrometry operating in the tandem mass spectrometry (MS/MS) mode. Samples were extracted into 5 M sodium chloride solution and partitioned into diethyl ether using Extrelut® 20 solid phase columns. Dried and concentrated extracts were derivatized with heptafluorobutyrylimidazole (HFBI) prior to determination by gas chromatography ion trap (GC/IT) MS/MS. Multiple reaction monitoring was used to monitor the parent/daughter ion transitions of the analytes and the stable isotope internal standard (3‐chloro‐1,2‐propandiol‐d 7 ). Calibration was linear over the range 5.0–500 pg/μL with correlation coefficients of 0.999 routinely obtained. The results showed that the method was capable of detecting 3‐MCPD below 5 μg/kg in HVP when analysed by GC/ITMS/MS. © 1997 John Wiley & Sons, Ltd.