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A rapid screening by liquid chromatography/mass spectrometry and fast‐atom bombardment tandem mass spectrometry of phenolic constituents with radical scavenging activity, from Krameria triandra roots
Author(s) -
Facino R. Maffei,
Carini M.,
Aldini G.,
De Angelis L.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199708)11:12<1303::aid-rcm966>3.0.co;2-j
Subject(s) - chemistry , chromatography , mass spectrometry , tandem mass spectrometry , fast atom bombardment , liquid chromatography–mass spectrometry , catechin , polyphenol , antioxidant , organic chemistry
Liquid chromatography/mass spectrometry (particle beam interface, electron impact ionization), fast‐atom bombardment mass spectrometry (FAB‐MS) (negative‐ion mode) and FAB‐tandem mass spectrometry (MS/MS) techniques were applied for the rapid detection/characterization of low‐to‐intermediate molecular weight polyphenol constituents of Krameria triandra roots with radical scavenging activity, this determined in a well established membrane model of lipid peroxidation (phosphatidylcholine liposomes). LC/MS analysis of the more lipophilic fractions of the drug (antioxidant activity: IC 50 , the concentration which gives 50% inhibition, 5.8 μg/mL petrol extract; IC 50 3.7 μg/mL chloroform extract) evidences at least 9 components belonging to the class of lignans; FAB‐MS/MS analysis of the butylacetate extract (IC 50 = 0.23μg/mL) caffeoyl esters, catechin‐glucosides, dimeric procyanidines and their corresponding phenylpropanoyd derivatives. © 1997 John Wiley & Sons, Ltd.