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Separation and characterization of the tryptic peptide mapping of recombinant bovine growth hormone by reversed‐phase high‐performance liquid chromatography electrospray mass spectrometry
Author(s) -
Chang Jen P.,
Kiehl Douglas E.,
Kennington Allison
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199708)11:12<1266::aid-rcm977>3.0.co;2-3
Subject(s) - chemistry , chromatography , trifluoroacetic acid , mass spectrometry , electrospray ionization , high performance liquid chromatography , peptide , protein mass spectrometry , reversed phase chromatography , electrospray , elution , acetonitrile , sample preparation in mass spectrometry , biochemistry
Reversed phase high‐performance liquid chromatography (RPHPLC) coupled with electrospray ionization mass spectrometry (ESI‐MS) was used to separate and characterize the peptides resulting from the tryptic cleavage of somidobove, a recombinant bovine growth hormone. The tryptic digestion of somidobove was carried out at room temperature for 15 hours. The tryptic peptides were separated on a Zorbax SB300 C8 column with trifluoroacetic acid – acetonitrile gradient elution and characterized by LC/ESI‐MS. Resulting single and combined peptide fragments were characterized by comparing the observed molecular mass with the calculated mass for these fragments. ©1997 John Wiley & Sons, Ltd.