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Identification of triacylglycerol positional isomers present in vegetable oils by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry
Author(s) -
Mottram Hazel R.,
Woodbury Simon E.,
Evershed Richard P.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199708)11:12<1240::aid-rcm990>3.0.co;2-5
Subject(s) - atmospheric pressure chemical ionization , chemistry , chromatography , high performance liquid chromatography , mass spectrometry , chemical ionization , vegetable oil , rapeseed , ionization , organic chemistry , food science , ion
High performance liquid chromatography in conjunction with atmospheric pressure chemical ionization mass spectrometry (HPLC/APCI‐MS) enables the positional isomers of individual triacylglycerols in mixtures to be identified. Application of HPLC/APCI‐MS to the analysis of soybean oil allowed thirty nine triacylglycerols and seven diacylglycerols to be positively identified. The compounds present were quantified and the distribution of fatty acids in the β‐position calculated. This distribution compared favourably with that obtained using a more conventional regiospecific lipase degradation of the oil. A survey of the acylglycerol components of a range of vegetable oils (blackcurrant, blue poppy seed, evening primrose, extra virgin olive, hazelnut, maize and rapeseed) carried out using HPLC/APCI‐MS is reported. ©1997 John Wiley & Sons, Ltd.