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Electrospray mass spectrometric analysis of poly(ethylene glycol)–protein conjugates
Author(s) -
Gioacchini Anna Maria,
Carrea Giacomo,
Secundo Francesco,
Baraldini Mario,
Roda Aldo
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199707)11:11<1219::aid-rcm989>3.0.co;2-v
Subject(s) - chemistry , electrospray , ethylene glycol , chromatography , molar mass , polymer , mass spectrometry , bioanalysis , electrospray mass spectrometry , organic chemistry
Proteins immobilized on polymer supports are extensively used to develop bioanalytical methods and the determination of the protein/polymer molar ratio is important both for quantifying the catalytic activity and calculating the diffusion rate of the substrates. In this paper a method, based on electrospray mass spectrometry, for characterizing subtilisin and lipase immobilized on chemically modified poly(ethylene glycol)‐5000 is described. The results obtained show that this technique is particularly effective. The information provided by it can be used to optimize the synthesis of these polymer–protein conjugates a well as to evaluate the efficiency of their separation and purification. © 1997 John Wiley & Sons, Ltd.