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Investigation of beam‐induced reactions occurring under fast‐atom bombardment conditions between triethanolamine and various phospholipids
Author(s) -
Boone Carolien M.,
Pergantis Spiros A.,
Heerma Wigger
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199707)11:11<1205::aid-rcm947>3.0.co;2-h
Subject(s) - chemistry , adduct , triethanolamine , fast atom bombardment , ion , diethanolamine , mass spectrum , mass spectrometry , deuterium , dissociation (chemistry) , analytical chemistry (journal) , organic chemistry , chromatography , physics , quantum mechanics
The generation of adduct ions when using a triethanolamine (TEA) matrix under conditions of fast‐atom bombardment has been investigated. In particular, phospholipids containing a free amino group react in the TEA matrix to form abundant adduct ions 26 and 42 mass units higher than the analyte pseudomolecular ions. The identity of these adduct ions was investigated using specific phosphatidylethanolamines and phosphatidylserine as well as 2‐aminoethyldihydrogenphosphate and 2‐aminoethylmonomethylphosphate as model compounds. Evidence obtained from positive‐ and negative‐ion high‐energy collision‐induced dissociation tandem mass spectra, high resolution mass spectra, deuterium labelling and experiments using diethanolamine/TEA mixtures revealed that the intensities of the adduct ions are dependent on the percentage of TEA in the matrix and on the polarity of the analyte. The nature of the adduct ions is best reflected by a structure in which a hydrogen atom of the free amino group is substituted by a vinyl (+26) or by an acetyl (+42) group. A mechanism for the adduct ion formation is proposed. © 1997 John Wiley & Sons, Ltd.