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Mass spectrometric identification of ligands selected from combinatorial libraries using gel filtration
Author(s) -
Dunayevskiy Yuriy M.,
Lai JanJi,
Quinn Cheryl,
Talley Frank,
Vouros Paul
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199707)11:11<1178::aid-rcm991>3.0.co;2-h
Subject(s) - chemistry , analyte , chromatography , size exclusion chromatography , mass spectrometry , capillary electrophoresis , combinatorial chemistry , identification (biology) , small molecule , peptide library , organic chemistry , peptide sequence , biochemistry , botany , biology , enzyme , gene
There is a constant search for a successful analytical methodology to provide high throughput screening of combinatorial libraries against biological targets for identification of active ligands. Solid‐phase screening assays offer faster isolation and identification of active analytes compared to the solution‐based iterative methods. On the other hand, shift of combinatorial research to the creation of soluble non‐peptide libraries, and limitations associated with the heterogeneous assays, creates a demand for a breakthrough technology for rapid and efficient screening of combinatorial libraries in solution. We demonstrated the efficient and rapid approach for selecting active ligands from a combinatorial mixture with subsequent identification of compounds by mass spectrometry. The procedure involves the use of a biological target molecule to physically isolate the active component in a mixture on a size exclusion medium. Then the ligands are identified using a combined liquid chromatography/capillary electrophoresis/mass spectrometry system. As a model system we used serum albumin and small molecules with different affinities to the protein. © 1997 John Wiley & Sons, Ltd.