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Application of Liquid Matrix‐assisted Laser Desorption/Ionization 4‐Sector Tandem Mass Spectrometry for the Analysis of Spider Toxin Acylpolyamines
Author(s) -
Fujita Tsuyoshi,
Itagaki Yasuhiro,
Hisaka Miki,
Naoki Hideo,
Nakajima Terumi,
Andriantsiferana Marta
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19970630)11:10<1115::aid-rcm908>3.0.co;2-d
Subject(s) - chemistry , mass spectrometry , analytical chemistry (journal) , mass spectrum , ionization , spider toxin , tandem mass spectrometry , tandem , ion , collision induced dissociation , soft laser desorption , ambient ionization , analyte , matrix assisted laser desorption electrospray ionization , matrix assisted laser desorption/ionization , desorption , chromatography , chemical ionization , electron ionization , organic chemistry , biochemistry , glutamate receptor , receptor , materials science , adsorption , composite material
We have applied a recently proposed liquid matrix‐assisted laser desorption/ionization (LMALDI) method on a 4‐sector tandem mass spectrometer equipped with an array detector at MS‐2. Although the ionizing laser beam is pulsed, the use of a liquid matrix can prolong the analyte ion current over 20 min without moving the laser irradiation spot and provide an ion current that is stable enough to acquire both mass spectra and collision‐induced dissociation (CID) spectra. Product ions, which originate from chemical background overlapping the precursor ions, were not observed in the MALDI‐CID spectra of acylpolyamines (below m/z 1000) unlike the case with FAB spectra. These LMALDI‐CID spectra are shown to provide detailed structural information of equivalent or higher equality compared with spectra obtained by using FAB‐high energy CID conditions on a 4‐sector tandem mass spectrometer. The advantages of the LMALDI‐CID method compared with FAB‐CID is discussed, together with the structure determination of new glutaminergic nerve blocker acylpolyamines obtained from Nephila madagascariencis spider venom.© 1997 John Wiley & Sons, Ltd.