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Improved resolution in the detection of oligonucleotides up to 60‐mers in matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry using pulsed–delayed extraction with a simple high voltage transistor switch
Author(s) -
Zhu Yongdong,
He Ling,
Srinivasan Jannavi R.,
Lubman David M.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19970615)11:9<987::aid-rcm949>3.0.co;2-j
Subject(s) - chemistry , mass spectrometry , resolution (logic) , time of flight mass spectrometry , extraction (chemistry) , analytical chemistry (journal) , ionization , oligonucleotide , chromatography , desorption , matrix (chemical analysis) , matrix assisted laser desorption electrospray ionization , matrix assisted laser desorption/ionization , dna , ion , adsorption , biochemistry , organic chemistry , artificial intelligence , computer science
Pulsed–delayed extraction using a simple high voltage transistor switch together with various sample purification approaches were used to enhance the resolution in matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) of oligonucleotides up to 60 bases long. This switch can provide a 0–3 kV voltage pulse with a 75 ns fall time. A resolution of 500–900 was typically observed for samples from 5‐mers to 60‐mers using pulsed–delayed extraction (PDE) with the switch described herein. The resolution deteriorated to <100 for oligonucleotides of ≥65‐mers. With the TOF acceleration region configuration used in this work, the resolution was found not to vary significantly over a delay range of 2–5 μs. As the DNA size increased to over 35‐mer, HPLC purification was required to retain the enhancement in resolution provided by PDE MALDI‐MS. © 1997 John Wiley &Sons, Ltd.