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Automated sample preparation using membrane microtiter extraction for bioanalytical mass spectrometry
Author(s) -
Janiszewski John,
Schneider Richard P.,
Hoffmaster Keith,
Swyden Monica,
Wells David,
Fouda Hassan
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19970615)11:9<1033::aid-rcm951>3.0.co;2-5
Subject(s) - chromatography , chemistry , analyte , bioanalysis , solid phase extraction , elution , extraction (chemistry) , sample preparation , mass spectrometry , solvent , high performance liquid chromatography , microtiter plate , cartridge , liquid chromatography–mass spectrometry , mechanical engineering , organic chemistry , engineering
The development and application of membrane solid phase extraction (SPE) in 96‐well microtiter plate format is described for the automated analysis of drugs in biological fluids. The small bed volume of the membrane allows elution of the analyte in a very small solvent volume, permitting direct HPLC injection and negating the need for the time consuming solvent evaporation step. A programmable liquid handling station (Quadra 96) was modified to automate all SPE steps. To avoid drying of the SPE bed and to enhance the analytical precision a novel protocol for performing the condition, load and wash steps in rapid succession was utilized. A block of 96 samples can now be extracted in 10 min., about 30 times faster than manual solvent extraction or single cartridge SPE methods. This processing speed complements the high‐throughput speed of contemporary high performance liquid chromatography mass spectrometry (HPLC/MS) analysis. The quantitative analysis of a test analyte (Ziprasidone) in plasma demonstrates the utility and throughput of membrane SPE in combination with HPLC/MS. The results obtained with the current automated procedure compare favorably with those obtained using solvent and traditional solid phase extraction methods. The method has been used for the analysis of numerous drug prototypes in biological fluids to support drug discovery efforts. © 1997 John Wiley & Sons, Ltd.