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The Enhancement of Polyphosphonate Salt Electrospray Mass Spectrometry and the High‐performance Liquid Chromatographic Separation of Triphosphate Esters by Diethylamine Treatment
Author(s) -
Ballantine James A.,
Games David E.,
Slater Paul S.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199704)11:6<624::aid-rcm743>3.0.co;2-c
Subject(s) - chemistry , salt (chemistry) , vanadate , diethylamine , inorganic chemistry , sodium , mass spectrometry , chromatography , nuclear chemistry , organic chemistry
Negative‐ion electrospray mass spectrometry was used to analyse the following polyphosphonated alkali metal salts: adenosine 5‐triphosphate disodium salt, cytidine 5‐triphosphate disodium salt, guanosine 5‐triphosphate trisodium salt, uridine 5‐triphosphate trisodium salt, inosine 5‐triphosphate trisodium salt, phytic acid dodecasodium salt hydrate, and coenzyme A trisodium salt. Before diethylamine (DEA) treatment, these compounds produced low intensity signals. The signals were generally a series of ion peaks which were identified as analyte acid ions with varying amounts of sodium adduct ions. With DEA treatment, there was a significant increase in the intensities of the acid ions and of those molecular fragments that retained a phosphonate group. All ion signals associated with sodium adduction were greatly reduced or eliminated. Preliminary results indicate that the polyphosphonated salts experience a sodium displacement mechanism similar to that previously reported for polysulphonated salts. © 1997 John Wiley & Sons, Ltd.