Premium
The Analysis of Some Corticosteroids and Corticosteroid Esters by Liquid Chromatography Coupled with Frit Fast‐atom Bombardment Mass Spectrometry
Author(s) -
Watson David G.,
Davidson Alistair G.,
Knight Barry I.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19970228)11:4<415::aid-rcm864>3.0.co;2-2
Subject(s) - chemistry , fast atom bombardment , frit , chromatography , mass spectrometry , corticosteroid , high performance liquid chromatography , methanol , mass spectrum , analytical chemistry (journal) , organic chemistry , medicine , materials science , metallurgy , surgery
A method utilizing a liquid chromatography/mass spectrometry method based on a Frit‐fast atom bombardment (FAB) interface was developed for the analysis of corticosteroids and corticosteroid esters. The method used a microbore octadecylsilane column with methanol + water as the mobile phase; 0.5% glycerol was incorporated into the mobile phase as the FAB matrix. Separation of a mixture, containing five corticosteroids was achieved. Full‐scan spectra were readily obtained for 20 ng of each steroid introduced into the instrument. © 1997 John Wiley & Sons, Ltd.