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Matrix‐assisted Laser Desorption/Ionization for Sequencing Single‐stranded and Double‐stranded DNA
Author(s) -
Taranenko N. I.,
Chung C. N.,
Zhu Y. F.,
Allman S. L.,
Golovlev V. V.,
Isola N. R.,
Martin S. A.,
Haff L. A.,
Chen C. H.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19970228)11:4<386::aid-rcm867>3.0.co;2-n
Subject(s) - chemistry , dna sequencing , mass spectrometry , desorption , template , dna , ionization , matrix (chemical analysis) , sequence (biology) , chromatography , matrix assisted laser desorption/ionization , computational biology , analytical chemistry (journal) , combinatorial chemistry , nanotechnology , biochemistry , ion , organic chemistry , materials science , adsorption , biology
The DNA sequence of a single‐stranded and double‐stranded template was determined. The templates were sequenced using the chain termination method and cycle sequencing method and detected by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. The sequencing products were analyzed successfully without the laborious and expensive methods for removal of the template. Direct sequencing of the double‐stranded template was achieved with minimal post‐reaction purifications, which could be extremely important for mutation analysis and clinical diagnosis. A systematic study of the mechanisms and kinetics of sequencing reactions was also performed. The details of this analysis and directions for future improvements of the quality of sequencing are presented. © 1997 John Wiley & Sons, Ltd. This paper was produced under the auspices of the US Government and it is therefore not subject to copyright in the US