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Electrospray Mass Spectrometric Characterization of a Corticosteroid Dimer
Author(s) -
Rendell Nigel B.,
Taylor Graham W.,
Vinson Gavin P.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19970131)11:2<219::aid-rcm740>3.0.co;2-o
Subject(s) - chemistry , dimer , electrospray , chromatography , mass spectrometry , electrospray ionization , electrospray mass spectrometry , organic chemistry
18‐Hydroxy‐11‐deoxycorticosterone forms a non‐polar dimer under acidic conditions. The dimer was purified by high performance liquid chromatography and further analysed by electrospray mass spectrometry. The purified dimer has a molecular weight of 656 Da with two free ketone groups and no free hydroxyl functions. The mass spectrometric data suggest a 20,21,20′,21′‐anhydro dimer structure for the dimer. Microbore high performance liquid chromatography/electrospray mass spectrometry of rat adrenal extracts indicated that both 18‐OH‐B and 18‐OH‐DOC were present, although no 18‐OH‐DOC dimer was detected. © 1997 John Wiley & Sons, Ltd.