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The Use of On‐line Capillary Electrophoresis/Electrospray Ionization with Detection via an Ion Trap Storage/Reflectron Time‐of‐flight Mass Spectrometer for Rapid Mutation‐site Analysis of Hemoglobin Variants
Author(s) -
Li Michael X.,
Wu JingTao,
Liu Lin,
Lubman David M.
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19970115)11:1<99::aid-rcm813>3.0.co;2-6
Subject(s) - chemistry , reflectron , mass spectrometry , capillary electrophoresis , ion trap , electrospray ionization , analytical chemistry (journal) , time of flight mass spectrometry , chromatography , time of flight , ion , electrospray , fragmentation (computing) , ionization , organic chemistry , computer science , operating system
Capillary electrophoresis/electrospray ionization using an ion trap storage/reflectron time‐of‐flight mass spectrometer detector (CE/ESI‐IT/reTOF) is used to provide a rapid and sensitive method for analyzing structural variants in the hemoglobin (Hb) β‐chain. The Hb α‐ and β‐chains are separated and the β‐chain is digested by trypsin. The digest is analyzed by CE/ESI‐IT/reTOF where a comparison of the total ion electrophorograms and mass spectra of the mutant and normal hemoglobins (Hbs) can detect the presence of a mutation site. In addition, collision‐induced dissociation in the vacuum interface — skimmer region can be used to pinpoint the identity of such a site. The unique capability of the CE/ESI‐IT/reTOF system for accurately detecting fast separations with narrow peaks that may be under 1 s full width at half maximum is demonstrated. The speed of this system is essential for resolution of the large number of peaks that are separated in a short time duration using CE separations. © 1997 John Wiley & Sons, Ltd.