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Two‐phase Matrix‐assisted Laser Desorption/Ionization: Matrix Selection and Sample Pretreatment for Complex Anionic Analytes
Author(s) -
Dale Michael,
Knochenmuss Richard,
Zenobi Renato
Publication year - 1997
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19970115)11:1<136::aid-rcm811>3.0.co;2-#
Subject(s) - chemistry , analyte , matrix assisted laser desorption electrospray ionization , desorption , matrix (chemical analysis) , matrix assisted laser desorption/ionization , ionization , soft laser desorption , sample preparation , sample (material) , analytical chemistry (journal) , chromatography , ion , organic chemistry , adsorption
Two‐phase matrices, consisting of a binary mixture of solid particulates and a low vapour pressure liquid, are shown to be effective for the production of both positive‐ion and negative‐ion laser desorption/ionization mass spectra (LDI‐MS). It was found that matrix selection can be guided by criteria developed previously for fast‐atom bombardment mass spectrometry. Two‐phase matrix‐assisted (MA)LDI‐MS of a variety of complex anionic analytes is presented. Sample pre‐treatment using ‘drop dialysis”, or exposure to an acid‐activated Nafion® cation‐exchange membrane, substantially enhances the quality of negative‐ion mass spectra. © 1997 John Wiley & Sons, Ltd.