Premium
Rapid Identification of Bacteria by Direct Matrix‐assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Whole Cells
Author(s) -
Krishnamurthy Thaiya,
Ross Philip L.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199612)10:15<1992::aid-rcm789>3.0.co;2-v
Subject(s) - chemistry , bacteria , mass spectrometry , chromatography , matrix (chemical analysis) , desorption , matrix assisted laser desorption/ionization , surface enhanced laser desorption/ionization , microorganism , yield (engineering) , ionization , sample preparation , analytical chemistry (journal) , ion , sample preparation in mass spectrometry , electrospray ionization , organic chemistry , biology , genetics , materials science , adsorption , metallurgy
Several characteristic ions were observed during the direct analysis of a variety of both gram‐negative and gram‐positive intact bacterial cells by the matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) technique. The entire process, involving absolutely no sample processing, could be completed in less than ten minutes. A number of specific biomarkers, generated reproducibly for each type of cell from the corresponding mass spectrum, permitted the identification, as well as the distinction, of pathogenic bacteria from their non‐pathogenic counterparts. In addition, individual strains of a specific organism could also be differentiated easily. Some of these biomarkers correspond to those observed earlier during the MALDI‐MS analysis of protein extracts of the same bacteria. This approach, which can yield valuable data for rapid classification and detection of microorganisms, represents a substantial breakthrough for rapid screening of environmental as well as biological samples