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High Performance Liquid Chromatography/Atmospheric Pressure Chemical Ionization‐Mass Spectrometry for the Determination of Carotenoids
Author(s) -
Clarke Philip A.,
Barnes Karen A.,
Startin James R.,
Ibe Frank I.,
Shepherd Martin J.
Publication year - 1996
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(199611)10:14<1781::aid-rcm748>3.0.co;2-d
Subject(s) - atmospheric pressure chemical ionization , chemistry , chromatography , high performance liquid chromatography , mass spectrometry , analyte , analytical chemistry (journal) , carotenoid , electrospray ionization , chemical ionization , liquid chromatography–mass spectrometry , electrospray , ionization , direct electron ionization liquid chromatography–mass spectrometry interface , elution , ion , organic chemistry , food science
The performance of electrospray ionization and atmospheric pressure chemical ionisation (APCI) for the analysis of carotenoids was investigated. On our instrument, APCI produced spectra which were readily interpreted, and the positive‐ion mode proved applicable to all of the compounds. The separation of nine compounds in under 14 minutes by gradient elution high‐performance liquid chromatography (HPLC) has been demonstrated. The performance characteristics of positive‐ion HPLC/APCI‐mass spectrometry have been investigated and documented for the determination of nine carotenoids. With the exception of β‐carotene, the precision of the method expressed as coefficient of variation ranged from 6 to 20%. Limits of detection ranged from 0.1 to 1.4 ng/μL dependent upon the analyte. The method was used to confirm the levels of carotenoids in UK Total Diet Survey samples.